\u57f9\u990a\u6db2\u306f\u3001\u7d30\u80de\u3084\u5fae\u751f\u7269\u3092\u57f9\u990a\u3059\u308b\u969b\u306b\u4f7f\u308f\u308c\u308b\u6db2\u4f53\u3067\u3001\u751f\u547d\u79d1\u5b66\u306e\u5206\u91ce\u3084\u533b\u7642\u7814\u7a76\u3067\u91cd\u8981\u306a\u5f79\u5272\u3092\u62c5\u3063\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u8a18\u4e8b\u3067\u306f\u3001\u57f9\u990a\u6db2\u306e\u57fa\u672c\u7684\u306a\u610f\u5473\u3084\u5f79\u5272\u3001\u7a2e\u985e\u306b\u3064\u3044\u3066\u8a73\u3057\u304f\u89e3\u8aac\u3057\u3001\u305d\u306e\u7528\u9014\u3092\u6df1\u6398\u308a\u3057\u307e\u3059\u3002<\/p>\n
\u57f9\u990a\u6db2\u3068\u306f\u3001\u7d30\u80de\u3084\u5fae\u751f\u7269\u3092\u80b2\u3066\u308b\u305f\u3081\u306b\u5fc5\u8981\u306a\u6210\u5206\u3092\u6eb6\u89e3\u3055\u305b\u305f\u6db2\u4f53\u3067\u3059\u3002\u7d30\u80de\u3084\u5fae\u751f\u7269\u304c\u751f\u80b2\u3057\u3001\u5897\u6b96\u3059\u308b\u305f\u3081\u306b\u6700\u9069\u306a\u74b0\u5883\u3092\u63d0\u4f9b\u3059\u308b\u305f\u3081\u3001\u5fc5\u8981\u306a\u6804\u990a\u7d20\u3001\u6210\u5206\u3001pH\u3001\u5869\u6fc3\u5ea6\u306a\u3069\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u4e3b\u306b\u751f\u7269\u5b66\u7684\u306a\u5b9f\u9a13\u3084\u88fd\u85ac\u3001\u533b\u7642\u5206\u91ce\u3001\u98df\u54c1\u5206\u91ce\u306a\u3069\u3067\u4f7f\u7528\u3055\u308c\u308b\u3053\u3068\u304c\u591a\u3044\u3067\u3059\u3002<\/p>\n
\u57f9\u990a\u6db2\u306e\u4e3b\u306a\u5f79\u5272\u306f\u3001\u7d30\u80de\u3084\u5fae\u751f\u7269\u304c\u6210\u9577\u30fb\u5897\u6b96\u3059\u308b\u305f\u3081\u306b\u5fc5\u8981\u306a\u6804\u990a\u7d20\u3092\u4f9b\u7d66\u3059\u308b\u3053\u3068\u3067\u3059\u3002\u307e\u305f\u3001\u7d30\u80de\u304c\u6b63\u5e38\u306b\u6a5f\u80fd\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u9069\u5207\u306apH\u3084\u6e29\u5ea6\u3001\u9178\u7d20\u6fc3\u5ea6\u3092\u4fdd\u3064\u3053\u3068\u304c\u91cd\u8981\u3067\u3042\u308a\u3001\u305d\u308c\u3089\u3092\u7dad\u6301\u3059\u308b\u305f\u3081\u306e\u6210\u5206\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u57f9\u990a\u6db2\u306b\u306f\u3001\u7d30\u80de\u306e\u4ee3\u8b1d\u3092\u4fc3\u9032\u3059\u308b\u305f\u3081\u306e\u6210\u5206\u3084\u3001\u6210\u9577\u56e0\u5b50\u3001\u30db\u30eb\u30e2\u30f3\u306a\u3069\u3082\u52a0\u3048\u3089\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n
\u57f9\u990a\u6db2\u304c\u9069\u5207\u3067\u306a\u3044\u5834\u5408\u3001\u7d30\u80de\u3084\u5fae\u751f\u7269\u306e\u6210\u9577\u304c\u6291\u5236\u3055\u308c\u3001\u5b9f\u9a13\u306e\u7d50\u679c\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u57f9\u990a\u6db2\u306f\u975e\u5e38\u306b\u91cd\u8981\u306a\u8981\u7d20\u3068\u306a\u308a\u3001\u7814\u7a76\u3084\u533b\u7642\u306e\u73fe\u5834\u3067\u306f\u614e\u91cd\u306b\u9078\u3070\u308c\u308b\u3079\u304d\u3067\u3059\u3002\u7279\u306b\u3001\u518d\u751f\u533b\u7642\u3084\u7d30\u80de\u6cbb\u7642\u306a\u3069\u306e\u5206\u91ce\u3067\u306f\u3001\u7d30\u80de\u306e\u54c1\u8cea\u3084\u6a5f\u80fd\u304c\u91cd\u8981\u306a\u305f\u3081\u3001\u57f9\u990a\u6db2\u306e\u54c1\u8cea\u7ba1\u7406\u304c\u6b20\u304b\u305b\u307e\u305b\u3093\u3002<\/p>\n
\u57f9\u990a\u6db2\u306b\u306f\u3055\u307e\u3056\u307e\u306a\u7a2e\u985e\u304c\u3042\u308a\u3001\u4f7f\u7528\u3059\u308b\u76ee\u7684\u3084\u57f9\u990a\u3059\u308b\u7d30\u80de\u3084\u5fae\u751f\u7269\u306b\u3088\u3063\u3066\u9069\u5207\u306a\u3082\u306e\u3092\u9078\u3076\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u4ee5\u4e0b\u306b\u4ee3\u8868\u7684\u306a\u57f9\u990a\u6db2\u306e\u7a2e\u985e\u3092\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n
\u7d30\u80de\u57f9\u990a\u6db2\u306f\u3001\u7d30\u80de\u306e\u5897\u6b96\u3084\u7dad\u6301\u306b\u5fc5\u8981\u306a\u6210\u5206\u304c\u542b\u307e\u308c\u305f\u6db2\u4f53\u3067\u3001\u4e3b\u306b\u52d5\u7269\u7d30\u80de\u3084\u690d\u7269\u7d30\u80de\u3092\u57f9\u990a\u3059\u308b\u305f\u3081\u306b\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002\u7d30\u80de\u57f9\u990a\u6db2\u306b\u306f\u3001\u30a8\u30c3\u30bb\u30f3\u30b7\u30e3\u30eb\u30a2\u30df\u30ce\u9178\u3001\u30d3\u30bf\u30df\u30f3\u3001\u7cd6\u5206\u3001\u5869\u985e\u3001\u305d\u306e\u4ed6\u306e\u6210\u9577\u56e0\u5b50\u304c\u542b\u307e\u308c\u3066\u304a\u308a\u3001\u7d30\u80de\u304c\u9069\u5207\u306b\u6a5f\u80fd\u3059\u308b\u305f\u3081\u306e\u74b0\u5883\u3092\u63d0\u4f9b\u3057\u307e\u3059\u3002<\/p>\n
\u4f8b: DMEM\uff08\u30c0\u30eb\u30d9\u30b3\u306e\u4fee\u6b63\u30a8\u30fc\u30b0\u30ea\u30fc\u30f3\u30e1\u30c7\u30a3\u30a2\uff09<\/p>\n
\u4f8b: RPMI-1640\uff08\u7d30\u80de\u5897\u6b96\u7528\u6db2\uff09<\/p>\n
\u5fae\u751f\u7269\u57f9\u990a\u6db2\u306f\u3001\u7d30\u83cc\u3084\u9175\u6bcd\u306a\u3069\u306e\u5fae\u751f\u7269\u3092\u80b2\u3066\u308b\u305f\u3081\u306b\u4f7f\u7528\u3055\u308c\u308b\u6db2\u4f53\u3067\u3001\u6804\u990a\u7d20\u3084\u6210\u5206\u304c\u8abf\u6574\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u5fae\u751f\u7269\u306e\u7a2e\u985e\u306b\u3088\u3063\u3066\u6700\u9069\u306a\u57f9\u990a\u6db2\u306e\u6210\u5206\u306f\u7570\u306a\u308a\u307e\u3059\u304c\u3001\u4e00\u822c\u7684\u306b\u70ad\u7d20\u6e90\uff08\u7cd6\uff09\u3001\u7a92\u7d20\u6e90\u3001\u7121\u6a5f\u5869\u3001\u30d3\u30bf\u30df\u30f3\u306a\u3069\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n
\u4f8b: LB\u57f9\u5730\uff08\u7d30\u83cc\u57f9\u990a\u7528\uff09<\/p>\n
\u4f8b: \u30b5\u30eb\u30e2\u30cd\u30e9\u83cc\u57f9\u990a\u6db2<\/p>\n
\u690d\u7269\u7d30\u80de\u3092\u57f9\u990a\u3059\u308b\u305f\u3081\u306e\u57f9\u990a\u6db2\u3067\u3001\u690d\u7269\u7d30\u80de\u304c\u6210\u9577\u3059\u308b\u305f\u3081\u306b\u5fc5\u8981\u306a\u6804\u990a\u7d20\u3084\u30db\u30eb\u30e2\u30f3\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u690d\u7269\u7d30\u80de\u57f9\u990a\u3067\u306f\u3001\u7d30\u80de\u5206\u88c2\u3084\u5668\u5b98\u5f62\u6210\u3092\u4fc3\u9032\u3059\u308b\u305f\u3081\u306b\u3001\u7279\u5b9a\u306e\u6210\u9577\u56e0\u5b50\u3084\u30db\u30eb\u30e2\u30f3\u304c\u91cd\u8981\u3068\u306a\u308a\u307e\u3059\u3002<\/p>\n
\u4f8b: MS\u57f9\u5730\uff08\u690d\u7269\u7d30\u80de\u57f9\u990a\u7528\uff09<\/p>\n
\u57f9\u990a\u6db2\u306b\u306f\u3001\u7d30\u80de\u3084\u5fae\u751f\u7269\u304c\u6700\u9069\u306b\u6210\u9577\u3059\u308b\u305f\u3081\u306b\u5fc5\u8981\u306a\u6210\u5206\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u308c\u305e\u308c\u306e\u6210\u5206\u304c\u3069\u306e\u3088\u3046\u306b\u4f5c\u7528\u3059\u308b\u304b\u3092\u7406\u89e3\u3059\u308b\u3053\u3068\u304c\u91cd\u8981\u3067\u3059\u3002<\/p>\n
\u57f9\u990a\u6db2\u306b\u542b\u307e\u308c\u308b\u6804\u990a\u6e90\u306f\u3001\u7d30\u80de\u3084\u5fae\u751f\u7269\u306e\u30a8\u30cd\u30eb\u30ae\u30fc\u6e90\u3068\u306a\u308b\u3082\u306e\u3067\u3059\u3002\u6700\u3082\u57fa\u672c\u7684\u306a\u6804\u990a\u6e90\u306f\u7cd6\u5206\u3067\u3001\u3053\u308c\u304c\u7d30\u80de\u306e\u30a8\u30cd\u30eb\u30ae\u30fc\u6e90\u3068\u306a\u308a\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001\u30b0\u30eb\u30b3\u30fc\u30b9\u3084\u4e73\u7cd6\u306a\u3069\u304c\u4f7f\u308f\u308c\u308b\u3053\u3068\u304c\u591a\u3044\u3067\u3059\u3002<\/p>\n
\u30a2\u30df\u30ce\u9178\u306f\u3001\u7d30\u80de\u304c\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5408\u6210\u3059\u308b\u305f\u3081\u306b\u5fc5\u8981\u306a\u6210\u5206\u3067\u3059\u3002\u7d30\u80de\u304c\u5206\u88c2\u3084\u6210\u9577\u3092\u3059\u308b\u969b\u306b\u3001\u30a2\u30df\u30ce\u9178\u306f\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u307e\u3059\u3002\u57f9\u990a\u6db2\u306b\u542b\u307e\u308c\u308b\u30a2\u30df\u30ce\u9178\u306f\u3001\u7d30\u80de\u306e\u7a2e\u985e\u306b\u5fdc\u3058\u3066\u8abf\u6574\u3055\u308c\u307e\u3059\u3002<\/p>\n
\u30d3\u30bf\u30df\u30f3\u3084\u30df\u30cd\u30e9\u30eb\u306f\u3001\u7d30\u80de\u306e\u4ee3\u8b1d\u3092\u30b5\u30dd\u30fc\u30c8\u3059\u308b\u305f\u3081\u306b\u5fc5\u8981\u306a\u6210\u5206\u3067\u3059\u3002\u30d3\u30bf\u30df\u30f3C\u3084\u30d3\u30bf\u30df\u30f3B\u7fa4\u3001\u30ab\u30eb\u30b7\u30a6\u30e0\u3001\u30de\u30b0\u30cd\u30b7\u30a6\u30e0\u306a\u3069\u304c\u542b\u307e\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u308c\u3089\u306f\u7d30\u80de\u306e\u6b63\u5e38\u306a\u6a5f\u80fd\u3084\u6210\u9577\u3092\u4fc3\u9032\u3057\u307e\u3059\u3002<\/p>\n
pH\u30d0\u30c3\u30d5\u30a1\u30fc\u306f\u3001\u57f9\u990a\u6db2\u306epH\u3092\u5b89\u5b9a\u3055\u305b\u308b\u305f\u3081\u306b\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002\u7d30\u80de\u3084\u5fae\u751f\u7269\u306e\u6d3b\u52d5\u306b\u306f\u3001\u4e00\u5b9a\u306epH\u7bc4\u56f2\u304c\u5fc5\u8981\u3067\u3042\u308a\u3001pH\u306e\u5909\u52d5\u304c\u6fc0\u3057\u3044\u3068\u6210\u9577\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u4e00\u822c\u7684\u306apH\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u306f\u3001HCO\u2083\u207b\uff08\u91cd\u70ad\u9178\uff09\u3084Hepes\u306a\u3069\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n
\u57f9\u990a\u6db2\u306f\u3055\u307e\u3056\u307e\u306a\u7528\u9014\u3067\u4f7f\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u7279\u306b\u751f\u547d\u79d1\u5b66\u3084\u533b\u7642\u5206\u91ce\u306b\u304a\u3044\u3066\u3001\u305d\u306e\u91cd\u8981\u6027\u306f\u8a08\u308a\u77e5\u308c\u307e\u305b\u3093\u3002<\/p>\n
\u57f9\u990a\u6db2\u306f\u3001\u533b\u85ac\u54c1\u306e\u7814\u7a76\u3084\u88fd\u9020\u306b\u304a\u3044\u3066\u4e0d\u53ef\u6b20\u3067\u3059\u3002\u4f8b\u3048\u3070\u3001\u7d30\u80de\u57f9\u990a\u3092\u4f7f\u7528\u3057\u3066\u3001\u30ef\u30af\u30c1\u30f3\u3084\u6297\u4f53\u306e\u88fd\u9020\u304c\u884c\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u57f9\u990a\u6db2\u304c\u9069\u5207\u3067\u306a\u3044\u3068\u3001\u751f\u6210\u3055\u308c\u308b\u533b\u85ac\u54c1\u306e\u54c1\u8cea\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u305f\u3081\u3001\u975e\u5e38\u306b\u614e\u91cd\u306b\u7ba1\u7406\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n
\u518d\u751f\u533b\u7642\u3084\u7d30\u80de\u6cbb\u7642\u306b\u304a\u3044\u3066\u3082\u3001\u57f9\u990a\u6db2\u306f\u6b20\u304b\u305b\u307e\u305b\u3093\u3002\u3053\u308c\u3089\u306e\u6cbb\u7642\u6cd5\u3067\u306f\u3001\u60a3\u8005\u81ea\u8eab\u306e\u7d30\u80de\u3084\u4ed6\u306e\u7d30\u80de\u3092\u57f9\u990a\u3057\u3066\u304b\u3089\u4f7f\u7528\u3059\u308b\u305f\u3081\u3001\u57f9\u990a\u6db2\u306f\u7d30\u80de\u306e\u5897\u6b96\u3068\u6a5f\u80fd\u306e\u7dad\u6301\u306b\u5927\u304d\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u307e\u3059\u3002<\/p>\n
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